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Celebrating Diversity Within the Sickle Cell Community: Commitment, Innovation, Practice
Saturday, October 13 • 8:30am - 8:45am
Pravastatin-Mediated Modulation Of Sickle Red Cell Adhesion Using Mouse Endothelial Cells In Vitro

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Abstract 

Authors:
Dr. Ogechukwu Egini- Interfaith Medical Center, Dr. Edouard Guillaume- Interfaith Medical Center, Dr. Eric Jaffe- Interfaith Medical Center, Dr. Xian-Cheng Jiang- SUNY Downstate Medical Center, Mrs. Stephanie Egini- Caters Plan for Healthy Living, Dr. Ugochi Ogu- Montefiore Medical Center, Ms. Miren Blackwood- Interfaith Medical Center, Dr. Antony Lixon- Interfaith medical Center

Background - Beside lipid-lowering effects of statins, they have been shown to decrease inflammatory markers in many trials. The PRINCE (Pravastatin inflammation/CRP evaluation) trial was a 24 week study in which volunteers were treated with Pravastatin and inflammatory markers were measured at end of study. A significant reduction in serum CRP levels in subjects was noted, independently of any changes in LDL cholesterol. Furthermore, simvastatin reduced vaso-occlusive pain and oral analgesic use in patients treated over months.
Objective - To determine whether Pravastatin reduced sickle cell adhesion to endothelial cells using mouse endothelial cells in culture.
Design - Randomized experimental study
Study subjects - 4 sickle cell anemia patients from Interfaith Medical Center sickle cell outpatient program and 3 normal controls were recruited from the outpatient clinic. Subject recruitment was approved by the Institutional Review Board.
Interventions - Demographic information and EDTA blood samples were collected. Red cells were centrifuged, washed and stained with Vybrant Dil fluorescent cell labeling solution. Mouse endothelial cells were cultured on well plates. They were then treated with either Pravastatin or placebo for 1 hour. Both normal and sickle red cells were then activated with 20nM epinephrine and then introduced into the endothelial cell culture medium treated with either Pravastatin or placebo. Incubation was done for 30 minutes.
Main measures - Comparison of the number of red cells adherent to the mouse endothelial cell membrane in culture.
Results - In the placebo and treatment groups, there was significant sickle red cell adhesion compared to normal cells (P value < 0.05). When adherent sickle red cells in both placebo and treatment groups are compared, there was significant sickle red cell adhesion to mouse endothelial cell membrane in the placebo group (p<0.05).
Conclusion - Pravastatin reduced sickle red adhesion to mouse endothelial cell membrane.

Speakers
OE

Ogechukwu Egini, MD

Medical Resident, Interfaith Medical Center


Saturday October 13, 2018 8:30am - 8:45am
Constellation B

Attendees (5)